Somatic Embryogenesis of Oil Palm (<i>Elaeis guineensis</i> Jacq.) from Bud Explants Using Suspension Culture

The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability produce entire plants without the need for gamete fusion. While embryo proliferation typically performed on solid media, this approach less efficient than liquid which both more cost-effective can lead better results. Thus, study aimed determine most effective combination growth regulators MS medium suspension propagation. Buds from served as initial explants, with embryogenic callus utilized culture. Bud explants were previously planted media 2,4-D BAP initiate primary was formed 97th 132nd days, D4B1 (80 mg/L 2,4-D; 2.5 BAP) containing callus. subcultured twice over 60-day period before induction. calli D3B1, D3B2, D4B0, between 90th 120th days. medium, again successfully initiated growth, hence it later used fresh weight increased during first second subcultures declined in third subculture. process repeated obtain coleoptilar embryos, them being 88th 115th days (40.7%). Somatic an way propagating use specific result formation embryos.